Standardized Phenotyping
Goal: Define a list and format of descriptors for use in breeding and germplasm evaluations that are applicable across breeding programs/genera/subfamilies/families of Rosaceae. Categories of descriptors have been identified as high priority:
- Disease Resistance
- Fruit Quality
- Productivity
- Abiotic Stress Resistance
Background: With a shared ancestral genome, the details of which are beginning to come to light from structural and comparative genomics research, the possibility exists to discover common gene networks underlying traits of interest to crops of the Rosaceae family. Techniques in genomics and bioinformatics are rapidly expanding for such comparative efforts, and the bottleneck is now the availability of readily comparable phenotypic data. Standardized phenotyping can be as “simple” as aligning data collection methods for a crop between two research locations, or between a breeding program and a genomics research study. It is more difficult to obtain phenotypic data for two different crops, particularly across subfamilies, that have the likelihood of being under common genetic control. Achieving this goal, however, holds the promise of strengthening Rosaceae-wide ties between breeding programs, germplasm collections, and genetics and genomics research.
Desirable characteristics of new/modified descriptors
- Heritable
- Easy to measure (time, cost logistics)
- Relevant to breeding programs (value to industry)
- Relevant to Research programs(biological basis for genomics and genetics studies)
- Objective data that can be collected uniformly across locations/time
- If subjective data, then include information on confidence level.
- Identify a set of reference accessions available internationally
Background Information:
- Bioversity descriptors (formally IPGRI) were developed by European crop-specific working groups. Download Rosaceae descriptors.
- GRIN (Germplasm Resources Information Network) descriptors: These have been developed for many Rosaceae crops (not all) by the Crop Germplasm Committees (current membership listings). The descriptors are crop specific and available online for apple, pear, fragaria, cherry and rubus.
- EURISCO descriptors: Another set of descriptor lists developed in the European community (download malus, pear, prunus).
- Trait Ontology listings: Trait ontologies have been developed for Gramene database and are therefore more oriented toward the grain crops. (Download summary spreadsheet)
- Summary Excel spreadsheet listing the Rosaceae descriptors from Bioversity, GRIN, and EURISCO sets. Download spreadsheet.
Discussion Groups held to discuss Rosaceae phenotypic descriptors
- American Society for Horticultural Science 7/07 Scotsdale, Arizona (download minutes)
- ISHS Fire Blight Conference 8/07 Portland, Oregon (download summary minutes, download detailed minutes)
- XVIII Eucarpia Congress, Fruit Section Meeting, 9/07 Zaragoza, Spain 9/07 (downlaod minutes)
- Germplasm and Phenotyping Committee Teleconference, 11/14/07, (download summary information [revised on 11-16-07], download meeting agenda)
Outcomes
- Recommend a list of priority descriptors and subsets of germplasm
- Coordinated phenotyping efforts
- Make data accessible via databases
Phenotype Descriptors
- Download the first draft of phenotype descriptors of Amygdaloideae
Primary Contacts
- Gayle Volk (gayle.volk@ars.usda.gov)
- Nahla Bassil (bassiln@science.oregonstate.edu)
- Committee Members
