Abbot Peach Project Description
Peach (Prunus persica) represents a model species for prunus and a reference species for Rosaceae, To develop an extensive Prunus EST database for identification and cloning of genes important to fruit and tree development, we generated 9984 high-quality expressed sequence tags (ESTs) from a peach cDNA library of developing fruit mesocarp. After assembly and annotation, a putative peach unigene set consisting of 3842 ESTs was defined.
Further annotation includes Gene Ontology (GO) classification and computational
detection of simple sequence repeats (SSR) and open reading frame
(ORF). Using core markers from
the general Prunus genetic map, BAC clones were anchored on the
genetic map providing
a framework for construction of a physical and a transcript map.
To develop a transcript map, 1236 ESTs from the putative peach
unigene set and
additional
68 peach cDNA clones were hybridized against the peach BAC library.
Hybridizing ESTs to genetically anchored BACs immediately localized
11.2% of the ESTs on
the genetic map.
October 29, 2002 : Abbott Peach ESTs Analyzed for SSRs
August 20, 2002 : Abbott Peach ESTs Submitted to GenBank
| Processing Summary | |
| Number of ESTs sequenced | 13331 | Number of successful sequences | 9984 | Average Success Rate | 74.9 % | Average Base Count | 502 | Average Number of HQ Bases | 347 | Average Phred Quality Value | 26 | Number of Contigs (CAP3 Assembly -p 95 -d 60 ) | 1306 | Number of Singletons | 3500 | Number of Putative Unigenes | 3843 |
