Peach Transcriptome Map

As an effort to develop an extensive Prunus EST database for identification and cloning of genes important to fruit and tree development, 9984 high-quality expressed sequence tags (ESTs) from a peach cDNA library of developing fruit mesocarp. After assembly and annotation, a putative peach unigene set consisting of 3842 ESTs was defined. Using core markers from the general Prunus genetic map, BAC clones are anchored on the genetic map, providing a framework for the construction of a transcript map. A transcript map was developed by hybridizing 1,236 ESTs from the putative peach unigene set. ESTs are anchored to genetic maps when they are hybridized to BACs that have previously been hybridized to genetic markers. ESTs are also anchored to genetic maps when the EST-hybridizing BACs belong to a BAC contig that contains genetically anchored BACs.

• Summary of the latest peach transcriptome map, Jan 2007 (Previous version Aug 2006)
  
  

  	Anchored by Shared BAC	Anchored by Shared BAC contig	Total
  No of genetically anchored ESTs	367 (367)	891 (382)	1258 (749)
  No of TxE anchored ESTs	216 (216)	796 (299)	1012 (515)

  
  
• View/Download the Peach Transcriptome Data
       An Excel spreadsheet with peach ESTs with the anchored map positions.
  
  
• Search ESTs by anchored map position
       Search ESTs by anchored map position including map name, linkage group and anchor type. Download the sequences of the search result in fasta format.
  
  
• Interactive TxE general prunus map with anchored BACs and ESTs
       Interactive map viewer that displays anchored BACs and ESTs to markers in each linkage group.
  
  
• View transcriptome map in CMap (ESTs anchored to TxE map)
  
  
• Prunus maps used for genetically anchored peach physical map development
  
  
• Publication:
       Horn R, et al. Candidate gene database and transcript map for peach, a model species for fruit trees.
       Theor Appl Genet. 2005 110:1419-28